Journal: bioRxiv

Article Title: The long non-coding RNA NEAT1 regulates the transcriptional landscape of cardiomyocytes

doi: 10.1101/2024.06.27.600932

Figure Lengend Snippet: (A) Experimental setup to detect the deregulated lncRNA in hearts subjected to HF, HF+AAV9-SERCA2a relative to Sham hearts (B) Microarray based profiling of RNA isolated from rat hearts subjected to Myocardial infarction and rescue by Serca2a gene therapy. (C) qRT-PCR of Neat1 from RNA isolated from rat ventricle after MI and MI+Serca2a gene therapy. (D) qRT-PCR for detecting the change in expression of ANP, β-MHC, Neat1, and Neat1.2 in primary neonatal rat ventricular myocytes (NRCM) challenged with 100 µM Isoproterenol for 48 h. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between both groups. (E) Neat1 expression levels detected by qRT-PCR and ejection fraction (EF%) throughout heart failure progression (TAC, transverse aortic constriction; n = 5–8). * p value < 0.05, t-tests were performed to compute statistical differences between Sham and TAC. (F) Neat1 expression levels and ejection fraction (EF%) throughout heart failure progression after subjecting mice to 15 mg/kg/day, 30mg/kg/day, and 50 mg/kg/day of isoproterenol for 10 days. * p value < 0.05, ** p value <0.01. t-tests were performed to compute statistical differences between saline and isoproterenol groups. (G) qRT-PCR for detecting the expression of total Neat1 and Neat1.2 in adult mice cardiomyocytes and adult mice non-myocyte fraction.

Article Snippet: Rat lncRNA microarray (4X44K, Arraystar) was performed in the heart samples isolated from the rats that were subjected to proximal coronary artery ligation (HF), HF+ SERCA2A gene therapy.

Techniques: Microarray, Isolation, Quantitative RT-PCR, Expressing, Saline